Histochemistry of officinal trichomes representing Lamiaceae family

K.N. Razarenova, E.V. Babushkina, P.D. Smirnov, O.V. Kostina, L.E. Muravnik
Keywords: Lamiaceae, glandular trichomes, capitate hairs, histochemistry.
Medicinskij al'manah, 2017, issue 3, pages 193-198.

Glandular trichomes localized on the leaves of some medicinal species of Lamiaceae have been studied

by light microscopy and histochemistry. The trichome morphology and dimensions are presented in the

text. The phenolic substances and terpenoids are found in the glandular trichomes. The secretory structures

have been shown to contain no sesquiterpene lactones. Histochemistry is a perspective method to

analyze glandular trichomes of medicinal plants containing essential oil.

1. Rastitel’nye resursy SSSR: Cvetkovye rasteniya, ikh khimicheskiy sostav,

ispol’zovanie. Sem. Asteraceae (Compositae). SPb. 1993. 352 s.

2. Rastitel’nye resursy SSSR: Cvetkovye rasteniya, ikh khimicheskiy sostav,

ispol’zovanie. Sem. Hippuridaceae – Lobeliaceae. SPb. 1991. 200 s.

3. Gosudarstvennaya Farmakopeya Rossiyskoy Federacii. XIII izd. T. 1. M. 2015.

400 s. – Rezhim dostupa: URL:

4. Gosudarstvennaya Farmakopeya SSSR. XI izd. Vyp. 2. M. 1989. 400 s.

5. Roskin G.I., Levinson L.B. Mikroskopicheskaya tekhnika. 3-e izd. M. 1957.

469 s.

6. Selivanov E.V. Krasiteli v biologii i medicine: Spravochnik. Barnaul. 2003. 40 s.

7. David R., Carde J-P. Coloration differentiele des inclusions lipidique et

terpeniques des pseudophilles du pin maritime au moyen du reactif nadi.

Comptes rendus de l'Acadеmie des Sciences. 1964. Vol. 258. Issue 12.

P. 1338-1340.

8. Geissmann T.A., Griffin T.S. Sesquiterpene lactones: acid-catalysed color

reactions as an aid in structure determination. Phytochemistry. 1971. Vol. 10.

Issue 10. P. 2475-2485.

9. Gutmann M. Improved staining procedures for photographic documentation

of phenolic deposits in semi-thinsections of plant tissue. Journal of Microscopy.

1995. Vol. 179. Issue 3. P. 277-281.

10. Morokhina S.L., Bobkova N.V., Sorokina A.A., Ermakova V.A. Morfologoanatomicheskoe

izuchenie novogo uspokoitel’nogo sbora. Farmaciya. 2012.

№ 3. S. 21-24.

11. Sorokina A.A., Agadzhanyan A.S. Morfologo-anatomicheskoe izuchenie

sedativnogo sbora s gibiskusom. Farmaciya. 2013. № 5. S. 17-19.

12. Werker E. Trichome diversity and development. Advances in Botanical

Research. 2000. Vol. 31. P. 1-35.

13. Maffei M., Chialva E., Sacco T. Glandular trichomes and essential oils in

developing peppermint leaves. I. Variation of peltate number and terpene

distribution within leaves. New Phytologist. 1989. Vol. 111. Iss. 4. P. 707-716.

14. Corsi G., Bottega S. Glandular hairs of Salvia officinalis: new data on

morphology, localization and histochemistry in relation to function. Annals of

Botany. 1999.Vol. 84. Issue 5. P. 657-664.

15. Shafiee-Hajiabad M., Novak J., Honermeier B. Characterization of glandular

trichomes in four Origanum vulgare L. accessions influenced by light reduction.

Journal of Applied Botany and Food Quality. 2015. Vol. 88. P. 300-307.

16. Chwil M., Nurzynska-Wierdak R., Chwil S., Matraszek R., Neugebauerovа J.

Histochemistry and micromorphological diversity of glandular trichomes in Melissa

officinalis L. leaf epidermis. Acta Scientiarum Polonorum Hortorum Cultus. 2016.

Issue 15(3). P. 153-172.

17. Tissier A. Glandular trichomes: what comes after expressed sequence tags?

The Plant Journal. 2012. Vol. 70. Issue 1. P. 51-68.

18. Kamasina V., Loziene K. The evaluation of phenotypic diversity of Thymus 

oblongifolius according to some anatomical characters and comparsion with parent

species. Acta Botanica Hungarica. 2009. Volume 51. Issue 1-2. P. 85-97.

19. Jia P., Liu H., Gao T., Xin H. Glandular trichomes and essential oil of Thymus

quinquecostatus. The Scientific World Journal. Vol. 2013. 8 p. (http://dx.doi.


20. Voirin B., Bayet C., Colson M. Demonstration that flavones aglycones

accumulate in the peltate glands of Mentha  piperita leaves. Phytochemistry.

1993. Vol. 34. № 1. P. 85-87.

21. Hossain M.A., Al-Hdhrami S.S., Weli A.M., Al-Riyami Q., Al-Sabahi J.N.

Isolation, fractionation and identification of chemical constituents from the leaves

crude extracts of Mentha piperita L grown in Sultanate of Oman. Asian Pacific

Journal of Tropical Biomedicine. 2014. Vol. 4. Suppl. 1. P. 368-372.

22. Masterova I., Uhrin D., Kettmann V., Suchy V. Phytochemical study of Salvia

officinalis L. Chemical Papers. 1989. Vol. 43. № 6. P. 797-803.

23. Lu Y., Foo L. Y. Flavonoid and phenolics glycosides from Salvia officinalis L.

Phytochemistry. 2000. Vol. 55. № 3. P. 263-267.

24. Kurkin V.A., Avdeeva E.V. Problemy standartizacii rastitel’nogo syr’ya i

preparatov, soderzhaschikh fenilpropanoidy. Farmaciya. 2009. № 1. S. 51-54.

25. Kurkin V.A., Kurkina T.V., Zapesochnaya G.G. Himicheskoe issledovanie travy

Melissa officinalis. Himija prirodnyh soedinenij. 1995. № 2. S. 318-320.

26. Shakeri A., Sahebkar A., Javadi B. Melissa officinalis L.– a review of its

traditional uses, phytochemistry and pharmacology. Journal of Ethnopharmacology.

2016. Vol. 188. P. 1-87. 27. Fortunato I.M., Avato P., Ruta C. Glandular hairs and essential oils in

micropropagated plants of Origanum vulgare L. Acta horticulturae 723. 2006.

P. 295-298.

28. Jovanovic A.A., Dordevic V.B., Zdunic G.M., Pljevljakusic D.S., Savikin K.P.,

Godevac D.M., Bugarski B.M. Optimization of the extraction process of polyphenols

from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted

techniques. Separation and Purification Technology. 2017. 51 p. (doi:http://dx.doi.


29. Han F., Ma G.-Q., Yang M., Yan L., Xiong W., Shu J.-C., Zhao Z.-D., Xu H.-L.

Chemical composition and antioxidant activities of essential oils from different

parts of the oregano. Journal of Zhejiang University-SCIENCE B (Biomedicine &

Biotechnology). 2017. Vol. 18. № 1. P. 79-84.

30. Nikoli'c M., Glamo'clija J., Ferreira I.C.F., Calhelha R.C., Fernandes A. et al.

Chemical composition, antimicrobial, antioxidant and antitumoractivity of Thymus

serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential

oils. Industrial Crops and Products. 2014. Vol. 52. P. 183-190.

31. Tohidi B., Rahimmalek M., Arzani A. Essential oil composition, total

phenolic, flavonoid contents, and antioxidant activity of Thymus species collected

from different regions of Iran. Food Chemistry. 2017. Vol. 220. P. 153-161.

32. Hallahan D.L. Monoterpenoid biosynthesis in glandular trichomes of

Labiatae plants. Advances in Botanical Research. 2000. Vol. 31. P. 77-120.

33. Saeb K., Gholamrezaee S. Variation of essential oil composition of Melissa

officinalis L. leaves during different stages of plant growth. Asian Pacific Journal of

Tropical Biomedicine. 2012. Vol. 2. Iss. 2. P. 54 7-549.

34. Farhat M.B., Jordаn M.J., Chaouch-Hamada R., Landoulsi A., Sotomayor J.

A. Phenophase effects on sage (Salvia officinalis L.) yield and composition of

essential oil. Journal of Applied Research on Medicinal and Aromatic Plants. 2016.

Vol. 3. Iss. 3. P. 87-93.

K.N. Razarenova, E.V. Babushkina, P.D. Smirnov, O.V. Kostina, L.E. Muravnik Histochemistry of officinal trichomes representing Lamiaceae family. Medicinskij al'manah 2017; (3): 193–198, http://dx.doi.org/10.21145/2499-9954-2017-3-193-198