Development of the method of quantitative determination of the amount of flavonoids in the herb of Bupleurum scorzonerifolium

S.A. Petukhova, V.M. Mirovich
Keywords: Bupleurum scorzonerifolium, flavonoids, spectrophotometry, quantification.
Medicinskij al'manah, 2017, issue 3, pages 203-205.

The technique of spectrophotometric quantitative determination of the sum of flavonoids in the grass of

the Bupleurum scorzonerifolium is developed. The maximum absorption of alcohol extract from the grass

of the Bupleurum scorzonerifolium with the addition of a 3% solution of aluminum chloride is at 412 nm and

coincides with the standard routine sample. Calculation of the percentage content of flavonoids was carried

out in terms of routine. With 9 independent determinations, the method error did not exceed ±2,70%.

Experiments with additives of the standard routine showed the correctness of the technique and the

absence of a systematic error. The content of the amount of flavonoids in the samples of raw materials is

from 3,88 to 5,52%.

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S.A. Petukhova, V.M. Mirovich Development of the method of quantitative determination of the amount of flavonoids in the herb of Bupleurum scorzonerifolium. Medicinskij al'manah 2017; (3): 203–205, http://dx.doi.org/10.21145/2499-9954-2017-3-203-205